SUBJECT: Auxotrophs after uv irradiation

DATE: 5/97

 

I am trying to set up a lab in which the students look for E. coli auxotrophs

on minimal media after uv irradiation for an intro level microbiology class. I

get plenty of cell death and hoped to find some mutants in the surviving cells,

but so far haven't found any. Has anyone done this successfully? What strain of

E. coli did you use? I can be reached through biolab or directly through email

kbaier@center.colgate.edu

Thanks, Kath

 

 

 

You might check out the KSU Yeas Genetics site....GENE.

 

http://www.phys.ksu.edu/gene/bb13.html

 

I helped to develop this lab if you have questions. It's one of our more

intense learning experiences each year.

 

Brad Williamson Olathe East High School

14545 W. 127th

bwilliam@sound.net Olathe, KS 66062

rbradw@ix.netcom.com 913-780-7120 fax: 913-780-7137

 

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