SUBJECT: Radioactives in teaching labs
DATE: 5/97
 
 
Im writing a paper on important litigation in biology laboratories in
higher education. One reviewer wants me to speak to the use of
radioactives. I have never heard of undergraduates using radioactives in
teaching labs. Are any of you aware of their use? Also, are there any
recent cases that you are aware of involving litigation from accidents in
teaching labs? Thanks for you help.
***************************************************************************
Thomas R. Pitzer--Lecturer/TA Coordinator
Dept. of Biological Sciences, OE 246, FIU
University Park, FL 33199
Office: (305) 348-1224
FAX: (305) 348-1986
Email: pitzert@fiu.edu
Homepage: http://www.fiu.edu/~pitzert/
***************************************************************************
 
 
I use tritium in a cell biology lab on the cell cycle. In Molecular
Biology I use P-32 for Southern blots and S-35 in sequencing. I know that
the trend is to stay away from radioactive materials. However, with small
classes, close supervision, and proper lab technique, I feel that there is
value in having students utilize radioisotopes. My students are primarily
college juniors and seniors. Thankfully, I have not heard of any
litigation surrounding the use of these materials at the undergraduate
level.
 
Weldon Jones
 
*****************************************************************************
C. Weldon Jones, Ph.D. email: w-jones@bethel.edu
Professor and Chair of Biology Tel.: (612) 638-6312
Bethel College Fax: (612) 638-6001
3900 Bethel Drive
St. Paul, MN 55112
 
 
We have used I125 in a radioimmunoassay for thyroid function, tridium for
cell cycle studies, and P32 and S35 in molecular biology labs. Some of our
molecular biology instructors have moved to nonradioactive methods and are
pleased with the results. It is unlikely that everyone will convert
however. We have not had any litigation problems, but we are regulated like
crazy. We have many hoops to jump through to get permission to use
radioactive material and every student using radioactive materials has to
participate in a short course presented by radiation safety. We are heavily
monitored because the NCR revoked the University's licensce a few years
agao when some techs in the medical school had spread P 32 all over the
place -the door handle, the counter, the bathroom door handle, etc. They
were even eating in the same room as their hot bench. Needless to say they
are no longer with us and the entire University remains on red alert, so to
speak.
 
 
Jim Bader
Department of Biology
Case Western Reserve University
Cleveland, Ohio 44106-7080
jxb14@po.cwru.edu
 
 
I've switched to nonisotopic detection, which doesn't
work nearly as well. But it works. I would have no problem using P-32
with advanced students [at low levels; I'd do the labeling reaxn], but I
DO have a problem with tritiated nucleotides! They're undetectable, so a
student can be covered in them, go out to dinner, and ingest large quantities,
along with others whose food he/she touches! P-32 at least you can detect
before they leave the lab, and deal with any contamination.
-Bob Moss
Wofford College
 
 
Thomas,
 
For years we have had a lab experiment in cell biology (usually a senior
level majors course) that uses radioactivity. As RSO I keep careful watch
on how the students are prepared for this two lab series and how we monitor
hands, cloths and the facilties/equipment. I've also had over the years a
lab in the molecular biology course that uses either 32P or 35S but we have
moved to non-radioactive systems in the past several years.
 
I'm wondering what issues you are addressing in your paper on litigation in
biology labs. Perhaps you could give us a sense by giving an outline of
the topics you are covering.
 
Earl Fleck
Professor and Chair
Department of Biology
Whitman College
Walla Walla, WA 99362
 
 
Thanks for the response. I should have been more complete with my original
email. I'm looking at negligence or other charges brought against teaching
laboratories as a result of accidents or other aspects of improper or
non-existent safety measures. Then I make recommendations to practice
primarily to assist instructors and administrators in creating and
maintaining safe labs with as much reasonable monitoring as possible. It
has a very legalese focus--it's not a safety guide.
 
From: "Thomas Pitzer" <pitzert@fiu.edu>
 

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